Dr.Brahma Deo Singh

Emeritus Professor
Ph D (University of Saskachewan) Canada
Contact Information:
Tel: +91-542-2368331 Fax: +91-542-2368693(O), +542-2575241 (R)
Email Id:brahmadsingh@gmail.com

 
Academic Qualifications:
 
S. No.DegreeInstitutionYear
1.High School U.P. Board of Education1961
2.IntermediateU.P. Board of Education1963
3.B.Sc.Allahabad University, Allahabad1965
4.M.Sc.Kanpur University, Kanpur1967
5.Ph. D.University of Saskatchewan Saskatoon, Canada1972
 
Brief writeup on area of specialization/awards/achievements:
 
Our current research focuses on two main areas of research

  1. Mapping of gene(s) for rust resistance in pea: Introduction of resistance genes through plant breeding remains an important and effective means of protecting plants from diseases. However, in order to make disease screening more reliable, it is desirable to use molecular markers to identify the resistance genes. We are trying to understand the mechanism of resistance to pea rust. Some of the components for rust resistance have been identified. Currently, we are in the process of mapping the gene (Ruf) using RAPD and ISSR markers in RIL mapping population.
  2. Development of in vitro regeneration, and slow-growth conservation techniques for some important crop plants: Plants have proved to be a treasure-house of biologically active substances. We are developing in vitro propagation protocol for large-scale multiplication of some of the important plant species. For example, tomato (Solanum lycopersicon), and pointed gourd (Trichosanthes dioica), which are important vegetable crops, and provide a variety of biologically active compounds in our diet. Some other plants like, Drumstick (Moringa olifera), Shalparni (Desmodium sp.) are also being studied. We also intend to develop slow-growth techniques, which will help in reducing time and efforts of the researchers for in vitro conservation of these species.
Awards:
  • Awarded First Prize of the Dr. Rajendra Prasad Puraskar of 1990 by the I.C.A.R., New Delhi, for the book "Padap Prajanan: Siddhant Evam Vidhiyan" written in Hindi.
  • Awarded First Prize of the Dr. Rajendra Prasad Puraskar of 1987 by the I.C.A.R., New Delhi for the book "Anuvanshiki" written in Hindi.
  • Gold Medals: Awarded Shri J.G. Srikhande and Shri Sita Ram Jaipuria Gold Medals for standing First in M.Sc. (Ag.) previous (1966) and Final (1967)Examinations, respectively.
  • Silver Medal: Awarded Swami Vivekananda Silver Medal for standing First in B.Sc. (Ag.) 1965.
  • Selected Junior Fellow of Indian Council of Agricultural Research, New Delhi, during M. Sc. (Ag.) 1965-1967.
  • Awarded National Scholarship for Standing First in B. Sc. (Ag.) 1965.
  • Awarded Merit-cum-Means Scholarship of Indian Council of Agricultural Research, New Delhi, during B. Sc. (Ag.) 1963-1965.
 
Projects Undertaken as PI/Co PI:
 

 
Sl. No.  Research Project  Funding Agency  Year 
1.  Increasing nitrogen fixing ability of legumes and exploring the possibility of transferring the ability of nitrogen fixation to non-leguminous crops   ICAR, New Delhi.   Sept.1975- Sept. 1980 
2.  Biochemical and genetic studies on genetic dwarfness in cereals  UGC, New Delhi.   Nov. 1981- Nov. 1984 
3.  Genetic analysis of symbiotic nitrogen fixation in legumes  UGC, New Delhi.   Sept.1983- Sept. 1986 
4.  Collection and evaluation of barley germplasm and breeding for nitrogen utilization efficiency under stress conditions  ICAR, New Delhi.   Nov. 1983- Nov. 1986 
5.  Introgression of genes for yield traits from Cicer reticulatum into Cicer arietinum (chickpea)  ICAR, New Delhi.   July 1987- July 1990 
6.  Development of chickpea lines resistant to Ascochyta and Alternaria blights through selection for toxin-resistant cells in vitro   DBT, New Delhi.   April 1992- March 1995 
7.  Genetic analysis of sprouting resistance and seedling vigour in spring wheat  ICAR, New Delhi.   April 1994- Sept. 1997 
8.  Identification and characterisation of rust resistance and mapping of genes for resistance in pea  ICAR, New Delhi.   Oct. 2000- Sept. 2003 

Research papers reviewed in books/reviews:

  • Four research papers reviewed by H.E. Street in Plant Tissue and Cell Culture. Blackwell Science Publishers, London, 1977.
  • Three research papers reviewed by J. Reinert and Y.P.S. Bajaj in Applied and Fundamental Aspects of plant cell, Tissue and Organ Culture. Springer-Verlag, Berlin, 1977.
  • Three research papers reviewed by R.M. Skirvin in Natural and Induced Variations in Tissue Culture. Euphytica 24: 241-246, 1978.
  • One paper reviewed by D.S. Letham, P.B. Godwin and T.J.V. Higgins in Phytohormones and Related Compounds: A Comprehensive Treatise. Elsevier, Amsterdam, 1978.
  • Thirteen research papers reviewed by M.W. Bayliss in Chromosomal Variations in Plant Tissue Culture. Int. Rew.Cytol. Supplement 11A: 144, Academic Press, New York, 1980.
  • One figure produced by M.W. Bayliss (Ibid).
  • One research paper reviewed by L. Rappaport in Plant Growth Hormones: Internal Control Points. Bot. Gaz. 141: 125-130, 1980.
  • Two research papers reviewed by M.J. Constantin in chromosomal Instability in Cell, Tissue Cultures and regenerated Plants. Environ. Exp. Bot. 21; 359-368, 1981.
  • Five research papers reviewed by S.S. Bhojwani and M.S. Razdan in Plant Tissue Culure: Theory and Practice (pp. 164-167). Elsevier Science Publishers, Amsterdam, 1983.
  • One plate of figures reproduced by S.S. Bhojwani and M.S. Razdan, pp.165 (ibid).
  • Two research papers reviewed by D.A. Evans, W.R. Sharp and H.P. Medina-Filho in Somaclonal and Gametoclonal Variation. Amer. J. Bot. 71b: 759-774, 1984.
  • Two research papers reviewed by R. Lal and S. Lal in Crop Improvement Utilizing Biotechnology (pp.20, 30 & 66). CRC Press Inc., Boca Raton, Florida 1990.
 
Any additional information:
 

Recognitions:

  • Elected Member, Plant Tissue Culture Association of India.
  • Nominated Member, Editorial Board of Indian Journal of Genetics and Plant Breeding (1990-92).
  • Elected Fellow, Indian Society of Genetics and Plant Breeding.
  • Member, National Organizing Committee of the International Conference on Biotechnology in Agriculture and Forestry, Feb. 15-18, 1993, C.S. Azad University of Agriculture and Technology, Kanpur, India.
  • Member, Task Force on Biotechnology, Department of Biotechnology, New Delhi (2003-2005).  

List of 10 major publications:

  1. Maggon, R., and Singh, B. D.1995. Promotion of bud regeneration by ABA in combination with BAP in epicotyl and hypocotyl explants of sweet orange (Citrus sinensis L. Osbeck). Scientia Horticulturae 63: 123-128.
  2. Tiwari, V., Singh, B. D. and Tiwari, K. N. 1998. Shoot regeneration and somatic embryogenesis from different explants of Brahmi [Bacopa monniera (L) Wettst.]. Plant Cell Reports 17: 538-543.
  3. Tiwari, K. N., Sharma, N. C., Tiwari, V., and Singh, B. D. 2000. Micropropagation of Centella asiatica (L.), a valuable medicinal herb. Plant Cell Tissue and Organ Culture 63: 179-185.
  4. Tiwari, V., Tiwari, K. N. and Singh, B. D. 2001. Comparative studies of cytokinins on in vitro propagation of Bacopa monniera. Plant Cell Tissue and Organ Culture 66: 9-16.
  5. Srivastava, K., Tiwari, K. N., Singh, R., Singh, B. D. and Jaiswal, H. K. 2001. Shoot regeneration from immature cotyledons of Cicer arietinum. Biologia Plantarum 44: 333-337.
  6. Singh, R., Srivastava, K., Jaiswal, H. K., Amla, D. V. and Singh, B.D. 2002. High frequency multiple shoot regeneration from decapitated embryo axes of chickpea and establishment of plantlets in the open environment. Biologia Plantarum 45: 503-508.
  7. Arun, B., Joshi, A. K., Chand, R. and Singh, B.D. 2003. Wheat somaclonal variants showing earliness, improved spot blotch resistance and higher yield. Euphytica 132: 235-241.
  8. Vijayalakshmi, S., Yadav, K., Kushwaha, C. Sarode, S.B., Srivastava, C.P., Chand, R. and Singh, B.D. 2005. Identification of RAPD markers linked to the rust (Uromyces fabae) resistance gene in pea (Pisum sativum). Euphytica 144: 265-274.
  9. Chand, R., Srivastava, C.P., Singh, B.D. and Sarode, S.B. (2006). Identification and characterization of slow rusting components in pea (Pisum sativum L.). Genetic Resources and Crop Evolution 53:219-214.
  10. Tiwari, V., Tiwari, K. N., and Singh, B.D. 2006. Shoot bud regeneration from different explants of Bacopa monniera (L.) Wettst. by trimethoprim and bavistin. Plant Cell Reports 25: 629-635.

Full list of Publications:
  1. Singh, B.D., Harvey, B.L., Kao, K.N., and Miller, R.A. 1972. Selection pressure in cell populations of Vicia hajastana cultured in vitro. Can. J. Genet. Cytol. 14: 65-70.
  2. Singh, B.D., Harvey, B.L. 1972. Canalization of ovary development in Hordeum vulgare L. Crop. Sci. 13: 564-547.
  3. Singh, B.D., Thomas, E.T. and Harvey, B.L. 1974. Effects of gibberellic acid on cell suspension cultures of higher plants. Indian J. Expl. Biol. 12: 213-215.
  4. Singh, B.D. 1974. Evidence of endoreduplication in callus cultures of Crepis suffreniana Steud. Indian J. Expl. Biol. 12: 289-291.
  5. Singh, B.D. 1974. Occurrence of endopolyploidy in mature seeds of Vicia hajasthana Grossh. Indian J. Expl. Biol. 12: 466-469.
  6. Singh, B.D., and., Harvey, B.L. 1975. Selection for diploid cells in suspension cultures of Haplopapus gracilis. Nature 253: 453.
  7. Singh, B.D., Harvey, B. L., Kao, K. N. and Miller, R. A. 1975. Karyotypic changes and selection pressure in Haplopapus gracilis suspension cultures. Can. J. Genet. Cytol. 17: 109-116.
  8. Singh, B.D., and Harvey, B.L. 1975. Cytogenetics studies on Haplopapus gracilis cells cultured on agar and liquid media. Cytologia 40: 347-354.
  9. Singh, B.D., and., Harvey, B.L. 1975. Effect of different 2,4-D concentrations on the cytogenetic behaviour of plant cells cultured in vitro. Biol. Plant. 17: 167-174.
  10. Singh, B.D. 1975. Phylogenetic relationship between Haplopapus gracilis and H. ravenii: Evidence from cell suspension cultures. Caryologia 28: 39- 43.
  11. Singh, B.D. 1975. Effects of physical conditions of medium on karyotypes of cell populations in vitro. Nucleus 18: 61-65.
  12. Singh, R.B., Singh, R.M. and Singh, B.D. 1975. Supplementing germplasm resources through mutagenesis. Majalah Batan 8: 80-85.
  13. Singh, B.D. and Harvey, B.L. 1975. Does 2,4-D induce mitotic irregularities in plant tissue culture? Experimentia 31: 785-786.
  14. Singh, R.M., Sriram, S. and Singh, B.D. 1975. Differential mitogenic effects of phytohemagglutinin in pea and barley. Indian J. Expl. Biol. 13: 512-513.
  15. Laxmi, V., Singh, R.B., Singh, R.M. and Singh, B.D. 1975. Induction of translocations and trisomics in pearl millet by gamma-rays and EMS. Indian J. Expl. Biol. 13: 460-464.
  16. Laxmi, V., Singh, R. B., Singh, B. D. and Singh, R. M. 1975. Meiotic anomalies induced by gamma-rays and EMS treatment in pearl millet. Indian J. Expl. Biol. 13: 466-467.
  17. Singh, B.D. 1975. A comprehensive study of cytogenetic effects of two culture media on suspension cultures of Haplopapus gracilis. Acta Bot. Indica 3: 98-103.
  18. Singh, B.D. 1976. Chemical composition of medium in relation to cytogenetic behaviour of Haplopapus gracilis callus cultures. Nucleus 19: 74-79.
  19. Singh, B.D. 1976. Comparison of cytogenetic effects of IAA, NAA and 2,4-D in callus cultures of Haplopapus gracilis (Nutt) Gray. Indian J. Expl. Biol. 14: 524-526.
  20. Singh, B.D., Singh,Y., Singh, R.M. and Singh, R.B. 1976. Reduction in anaphase bridge frequency by 2,4-D in irradiated and normal wheat (Triticum aestivum L.) root tips. Indian J. Expl. Biol. 14: 526-527.
  21. Sharma, G.S., Singh, R.B., Singh, B.D. and Singh, R.M. 1976. EMS induced polygenic mutation for yield and yield components in spring bread wheat (Triticum aestivum L.). Cereal Res. Commun. 4: 321-333.
  22. Singh, R.B., Laxmi, V., Singh, B.D. and Singh, R.M. 1976. Induction of resistance to diseases in pearl millet inbreds by gamma-rays and EMS treatments. J. Cytol. Genet. 11: 122-126.
  23. Singh, R. M. Singh, R. B., Singh, B. D. and Laxmi, V. 1976. Quantitative variation induced by gamma rays and EMS treatments. J. Cytol. Genet. 11: 127-133.
  24. Venkateswarlu, S., Singh, R.M. Singh, R.B. and Singh, B.D. 1976. An induced variation for inflorescence in Cajanus cajan. J. Cytol. Genet. 11: 134-135.
  25. Singh, B.D. 1976. Effects of IAA, NAA and 2,4-D on cytogenetic behaviour of Haplopapus gracilis callus cultures. Caryologia 29: 447-455.
  26. Singh, R.B., Singh, B.D., Lakshmi V. and Singh, R.M. 1977. Meiotic behaviour of spontaneous and mutagen induced partial desynaptic plants in pearl millet. Cytologia 42: 41-47.
  27. Singh, B.D., Singh, R.B., Singh, R.M. and Laxmi, V. 1977. Effects of physical and chemical mutagens and male sterile cytoplasm on chiasma frequency in pearl millet inbreds and hybrids. Indian J. Expl. Biol. 15: 355-358.
  28. Singh, R.B., Singh, B.D., Singh, R.M. and Laxmi, V. 1977. Meiosis in radiation induced triploid and tetraploid plants of pearl millet. Cytologia 42: 633-637.
  29. Singh, B.D., Singh, Y., Singh, R.B., Singh, R.M., Sarma, N.D.R.K. and Singh, J. 1977. Reduction in anaphase bridge frequency by 2,4-D in irradiated and normal wheat (Triticum aestivum L.) root-tips. Indian J. Expl. Biol. 15: 688-691.
  30. Singh, B.D., Singh, Y., Singh, R.B., Singh, V.P., Singh, and Bhatnagar, P.S. 1977. Cytogenetic effects of seed treatment with IAA, NAA and 2,4-D in barley (Hordeum vulgare L.). Indian J. Expl. Biol. 15: 1105-1108.
  31. Singh, B.D. 1977. Parasexual hybridization in crop improvement. In: Parasexual Mechanism and Distant Hybridisation in Crop Improvement (ed. Chowdhary, J. B.), pp. 39-42.
  32. Singh, B.D. 1977. Application of embryo culture technique with special reference to crop improvement. In: Parasexual mechanism and Distant Hybridisation in Crop Improvement (ed. Chowdhary, J. B.), pp. 43-48.
  33. Singh, B.D., Singh, Y., Singh, J., Singh, R.B., and Singh, R.M. 1978. Meiotic irregularities induced by insecticide treatments in barley (Hordeum vulgare). J. Cytol. Genet. 13: 125-128.
  34. Das, K., Singh, B.D., Singh, R.B., Singh, J., and Singh, R.M. 1978. Cytological aberrations induced by Lasso, Machete, and Stam F-34 in barley (Hordeum vulgare). Indian J. Expl. Biol. 16: 446-449.
  35. Singh, B. D., Singh, V.K., Singh, R.B., and Singh, R.M.1978. Effects of buffers on carrot callus and suspension cultures. Indian J. Expl. Biol. 16: 842-844.
  36. Singh, R.M., Agarwal, P.K., Singh, J., Singh, B.D. and Singh, R.B.1978. Effects of storage of EMS and HA treated seeds on mutation frequency in barley (Hordeum vulgare). Indian J. of Expl. Biol. 16: 1067-1069.
  37. Singh, V. P., Singh, B. D., Singh, R. B., Dhar, B., Singh, R. M. and Srivastava, J. S. 1978. Effects of herbicide, Alachlor on growth and nitrogen fixation in cyanobacteria and rhizobia. Indian J. Expl. Biol. 16: 1325-1327.
  38. Venkateswarlu, S., Singh, R.M., Singh, R.B., and Singh, B.D. 1978. Radiosensitivity and frequency of chlorophyll mutations in pigeon pea. Indian J. Expl. Biol. 38: 90-94.
  39. Singh, R.B., Singh, B.D., Singh, R.M., and Laxmi, V. 1978. Seedling injury pollen sterility and morphological mutation induced by gamma-rays and EMS in pearl millet. Indian J. Expl. Biol. 38: 380-389.
  40. Dhar, B., Singh, B.D., Singh, R.B., Singh, R.M., Singh, V.P., and Srivastava, J.S. 1978. Isolation characterization of a virus (RL1) infective on Rhizobium leguminosarum. Arch. Microbiol. 119: 263-267.
  41. Singh, B.D., Singh, R.B., Singh, R.M., Singh, Y. and Singh, R.P. 1978. Endogenous GA levels and response to exogenous GA3 of some wheat strains. In: Proc. Fifth Intl. Wheat Genet. Symp. Vol. I (ed. Ramanujam, S.). pp. 510-513, Indian Soc. Genet. Plant Breed., New Delhi.
  42. Singh, V.P., Singh, R.B., Singh, B.D., Singh, R.M., Dhar, B., and Srivastava, J.S. 1978. Toxicity of butachlor to nitrogen fixing microorganisms. Beitr. Biol. Pflanzen. 54: 227-237.
  43. Singh, B.D., Singh, R.B., Singh, R.M., Singh, Y. and Singh, J. 1979. Effects of insecticides on germination, early growth, and cytogenetic behaviour of barley (Hordeum vulgare). Environ. Expl. Bot. 19: 127-132.
  44. Srivastava, C.P., Singh, Y., Bhatnagar, P.S., Singh, R.M., Singh, B.D. and Singh, R.B. 1979. Effect of exogenous GA3 on height, internode number and internode length of tall and dwarf strains of rice. Indian J. Agric. Sci. 49: 785-789.
  45. Singh, B.D., Singh, Y., Singh, R.B., and Singh, R.M. 1979. Differential response of tall and dwarf strains of rice to exogenous gibberellic acid (GA3). I1 Riso 23: 115-117.
  46. Dhar, B., Singh, B.D., Singh, R.B., Srivastava, J.S. Singh, V.P. and Singh, R.M. 1979. Occurrence and distribution of rhizobiophages in Indian soils. Acta Microbiol. Polonica 28: 319-324.
  47. Singh, B.D., Singh, R.B. and Singh, R.M. 1979. Photosynthetic efficiency: Prospects of genetic modification. Ann. Rev. Plant Sci. 1: 173-182.
  48. Singh, B.D., Singh, R.P., Singh, R.B, and Singh, R.M. 1979. Organogenesis in mung (Vigna radiata var. aureus) from macerated shoot tips. In: Plant Tisssue Culture, Genetic Manipulation and Somatic Hybridisation of Plant Cells (eds. Rao, P. S., Heble, M. R. and Chadha, M. S.), pp. 335-341, BARC, Bombay.
  49. Singh, R.B., Dhar, B. and Singh, B.D. 1980. Morphology and general characteristics of viruses active against cowpea Rhizobium CB 756 and 32H1. Arch. Virol. 64: 17-24.
  50. Singh, R.K., Singh, R.B., Singh, R.M. and Singh, B.D. 1980. Nitrogen fixation by nitrate reductase deficient mutants of Rhizobium japonicum. Indian J. of Expl. Biol.18: 1165-1167.
  51. Dhar, B., Singh, B.D., Singh, R.B., Srivastava, J.S. and Singh, R.M. 1980. Seasonal incidence of rhizobiophages in soils around Varanasi. Indian J. of Expl. Biol. 18: 1168-1170.
  52. Srivastava, J.S., Singh, B.D., Dhar, B., Singh, V.P., Singh, R.B., and Singh, R.M. 1980. Use of streptomycin resistance and phage sensitivity as markers in competition studies with Rhizobium leguminosarum. Indian J. of Expl. Biol. 18:1171-1173.
  53. Dhar, B., Singh, B.D., Singh, R.M. Singh, V.P. and Singh, R.B. 1980. Isolation and characterisation of antibiotic resistant mutants of cowpea Rhizobium 32H1. Indian J. of Expl. Biol. 18:1392-1397.
  54. Singh, R.M., Singh, A.K., Singh, R.B., and Singh, B.D. 1980. Chlorophyll mutations induced by seed treatment with certain insecticides in barley (Hordeum vulgare). Indian J. of Expl. Biol. 18:1396-1397.
  55. Srivastava, J.S., Murty, B.K., Singh, B.D., Singh, R.B., Singh, R.M. and Dhar, B. 1980. Competition between inoculated cowpea Rhizobium and native Rhizobium population. Beitr. Biol. Pflanzen 55: 147-155.
  56. Sahu, G.R., Mukherjee, P., Singh, B.D. and Singh, R.B. 1980. Induced polygenic variability in safflower (Carthamus tinctorius L.). J. Cytol. Genet. 15: 81-85.
  57. Singh, R.P., Singh, B.D. and Singh, R.B. 1981. Effect of buffers and pH on growth and protein content of carrot (Daucus carota L.) in liquid shake culture. Proc. Indian Acad. Sci. 90: 183-188.
  58. Singh, B.D., Singh, R.P. and Singh, R.B. 1981. Endogenous gibberellin and amylase activity in tall and dwarf strains of rice (Oryza sativa). Experientia 37: 363-364.
  59. Singh, R.P., Singh, R.B. and Singh, B.D. 1981. Biochemical basis of dwarfism in some rice (Oryza sativa) strains. Indian J. Expl. Biol. 19: 356-358.
  60. Mukherjee, P., Dwivedi, S.L., Singh, B.D., and Sahu, G.R. 1981. Genetic divergence and character associations in pearl millet. Indian J. Agric. Sci. 51: 69-72.
  61. Singh, R.B., Dhar, B., Singh, B.D., Singh, R.M., and Srivastava, J.S. 1981. Rhizobiophages in Indian soils: distribution, morphology and geenral characteristics. In: Current perspectives in Nitrogen Fixation (eds. Gibson, A. H. and Newton, W. E.). pp. 438. Australian Academy of Sciences, Canberra.
  62. Singh, B.D., Srivastava, J.S., Singh, R.B., Dhar, B., and Singh, V.P. 1981. Nodulation of inoculants of pea and cowpea Rhizobium in competition with native Rhizobia. In: Current perspectives in Nitrogen Fixation (eds. Gibson, A. H. and Newton, W. E.). pp. 513. Australian Academy of Sciences, Canberra.
  63. Singh, B.D. 1981. Origin of aneuploid variation in tissue cultures of Haplopappus gracilis and Vicia hajastana. Caryologia 34: 337-343.
  64. Srivastava, J.S., Singh, B.D., Tewari, V., Singh, R.M. and Singh, S. 1981. Effect of azide on cowpea Rhizobium strain 32H1. Microbios Lett. 18: 71-76.
  65. Tewari, V., and Singh, B.D. 1981. Probable interchanges induced by gamma-rays in pigeon pea (Cajanus cajan (L.) Millsp.). Legume Res. 4: 56-58.
  66. Tewari, V., Singh, R.B., and Singh, B.D. 1981. Colchicine induced tetraploids in pigeon pea (Cajanus cajan (L.) Millsp.). Legume Res. 4: 83-89.
  67. Tyagi, P.S., Singh, B.D., Jaiswal, H.K., Agnihotri, A. and Singh, R.M.1982. Path analysis of yield and protein content in chickpea. Indian J. Agric. Sci. 52: 81-85.
  68. Singh, R. P., Singh, B. D., Jaiswal, H. K., Singh, R. M. and Singh, R. B. 1982. Organogenesis in callus cultures of chickpea. Indian J. Agric. Sci. 52: 86-90.
  69. Tyagi, P.S., Singh, B.D., Jaiswal, H.K. 1982. Studies of the genetics of protein content in chickpea. Intl. Chickpea Newslett. 6: 6-8.
  70. Singh, B.D., Rao, G.S.R.L., and Singh, R.P.1982. Polyphenol accumulation in callus cultures of cowpea (Vigna sinensis). Indian J. of Expl. Biol. 20: 387-389.
  71. Jaiswal, H.K., Singh, B.D., Sastry, E.V.D., Tyagi, P.S. and Singh, R.M. 1982. Promotion of germination by gibberellic acid in some wild species of Cicer. Pulse Crops Newslett. 2: 32-33.
  72. Singh, B. D. and Singh, Y., 1982. Dwarf mutants of rice (Oryza sativa) with partial block in gibberellin utilisation. Biochem. Phisiol. Pflanzen 177: 789-791.
  73. Singh, V.P., Yadav, R.D.S., Singh, R.M., Singh, B.D. and Singh, R.B. 1982. Note on a golden yellow seed coat color mutant of mung bean (Vigna radiata (L.) Wilczek) induced by gamma-irradiation. Legume Res. 5: 111-114.
  74. Singh, R.M., Singh, J., Singh, J.P., Singh, R.B., and Singh, B.D. 1982. Modification of mutation frequency and spectrum in wheat and barley. In: Advances in Cytogenetics and Crop Improvement (eds. Singh, R. B., Singh, R. M. and Singh, B. D), pp. 257-266. Kalyani Publishers, New Delhi.
  75. Singh, B.D., Singh, R.B., Singh, R.M. 1982. Cytogenetic instability of plant tissue cultures. In: Advances in Cytogenetics and Crop Improvement (eds. Singh, R. B., Singh, R. M., and Singh, B. D), pp. 257-266 Kalyani Publishers, New Delhi.
  76. Singh, R. K., Singh, B. D. and Singh, H. N. 1983. Inhibition of photosystem II of nitrogen-fixing blue-green alga Nostoc linckia by rice field herbicide benthiocarb. Z. Allgemin. Mikrobiol. 23: 435-441.
  77. Singh, R.K., Singh, Ramesh K. and Singh, B.D. 1983. Transfer of nitrate reductase genes of cyanobacterium Nostoc muscorum into Rhizobium japonicum. J. Gen. Microbiol. 129: 2531-2534.
  78. Singh, R.P., and Singh, B.D. 1984. Promotory effect of glutamine on root regeneration from callus cultures of mung. Curr. Sci. 53: 148-149.
  79. Singh, B.D., Jaiswal, H.K., Singh, R.M. and Singh, A.K. 1984. Isolation of early flowering recombinants from the interspecific cross between Cicer arietinum and C. reticulatum. Intl. Chickpea Newslett. 11: 14-16.
  80. Jaiswal, H.K., Singh, B.D. and Singh, R.M. 1984. Possible origin of desi type chickpea (Cicer arietinum L.) through introgression of C. reticulatum genes. Intl. Chickpea Newslett. 11:18-20.
  81. Singh, B.D. 1984. Variation in chromosome number and structure in plant cells during in vitro culture. In: Proc. Int. Symp. Plant Tissue and Cell Culture- Application to crop improvement (eds. Novak, F. J., Havel, L. and Dolelzel, J.), pp. 305-314. Olomouc, Czechoslovakia, Sept. 24-29, 1984.
  82. Singh, R.P., and Singh, B.D., Singh, R.M. and Jaiswal, H.K. 1985. Genotypic differences in callus growth and organogenesis in green gram. Indian J. Agric. Sci. 55: 612-615.
  83. Singh, B.D., Singh, R.M., Murty, B.K. and Singh, R.B. 1985. Combining ability analysis of nodulation characters in mung bean (Vigna radiata (L.) Wilczek). Indian J. Genet. 45: 271-277.
  84. Singh, R.P., Singh, B.D. and Singh, R.M. 1986. Organ regeneration in mung bean callus cultures. In: Genetics and Crop Improvement (eds. Gupta, P. K. and Bahl, J. R.), pp. 387-393. Rastogi and Co., Meerut.
  85. Jaiswal, H.K., Singh, B.D., Singh, R.M., Singh, A.K. 1986. Introgression of genes for yield and yield traits from Cicer reticulatum into Cicer arietinum. Intl. Chickpea Newslett. 14: 5-8.
  86. Singh, B.D. 1986. Genetics of nitrogen fixation in legumes. In: Pigeonpea Genetics and Improvement (ed. Tikka, S. B. S.), pp. 173-204. Sharda Publ. Academy, Sidhpur.
  87. Singh, B.D. 1986. Role of tissue culture in improvement of pulse crops. Narendra Dev. J. Agric. Res. 1: 88-95.
  88. Tewari, V. and Singh, B.D. 1986. Cytological behaviour of autotetraploids in pigeonpea (Cajanus cajan (L.) Millsp.). Narendra Dev. J. Agric. Res. 1: 163-166.
  89. Tewari, V., Singh, B.D. and Sharma, G.S .1986. Combining yield traits in certain rust resistant lines of wheat. Madras Agric. J. 73: 216-220.
  90. Singh, R.P., Tewari, V., Singh, B.D. and Singh, R.M. 1986. Inheritance of morphological traits in an interspecific cross Cicer scarabaeoides (L.) Benth x Atylosia sericea (L.) Benth ex. Baker). Pulse Crops Newslett. 6: 8-10.
  91. Dhar, B., Ramakrishna, K., Singh, B.D. and Singh, R.M. 1987. Morphology and biological properties of phage RL4 of Rhizobium leguminosarum SU391. Indian J. of Expl. Biol. 25: 38-41.
  92. Jaiswal, H.K., Singh, B.D. and Singh, R.M., 1987. Improvement of chickpea (Cicer arietinum) through introgression of genes from Cicer reticulatum. Indian J. Agric. Sci. 57: 880-883.
  93. Tewari, V. Singh, B.D. and Sharma, G.S. 1987. Genetics of leaf rust resistance and combining ability for yield in wheat. Narendra Dev. J. Agric. Res. 2: 121-125.
  94. Singh, B.D. and Murty, B.K. 1988. Genetic analysis of nitrogen fixation traits in green gram (Vigna radiata). Indian J. Agric. Sci. 58: 171-175.
  95. Bhatnagar, P.S., Tewari, V. and Singh, B.D. 1988. Host-Rhizobium symbiotic interaction in mungbean (Vigna radiata (L.) Wilczek). Genet. Agr. 42: 161-168.
  96. Singh, I. S., Singh, B. D., Singh, R. P. and Singh, K. K., 1988. Interrelationships of yield and its components in F3 progenies of a cross in mungbean. Crop. Improv. 15: 146-150.
  97. Singh, R.P., Singh, B.D., Singh, R.N. and Singh, I.S. 1989. Comparison of bulk and single seed descent methods in two mungbean crosses. Crop. Improv. 16: 24-28.
  98. Singh, V.K., Singh, B.D., Shahi, J.P. and Singh, R.P. 1989. Estimates of genetic variances in a population of maize. Crop. Improv. 16: 29-33.
  99. Singh, R.P. and Singh, B.D. 1989 Recovery of rare interspecific hybrids of gram Cicer arietinum x C. cuneatum through tissue culture. Curr. Sci. 58: 874-876.
  100. Jaiswal, H.K., Singh, B.D. 1989. Analysis of gene effects for yield and certain yield traits in crosses between Cicer arietinum L. and C. reticulatum Ladz. Indian J. Genet. 49: 9-17.
  101. Singh, I.S., Singh, B.D., Singh, K.K. and Singh, R.P. 1989. Character association in selected F3 progenies of cross K851 x BN of mungbean (Vigna radiata (L.) Wilczek). Narendra Dev. J. Agric. Res. 4: 206-211.
  102. . Singh, B.D. and Jaiswal, H.K. 1990. Introgression of genes from Cicer reticulatum into chickpea. In: Trends in Crop Improvement (eds. Bahl, R. K. and Maherchandani, N.), pp. 46-54. Plant Science Colloquium, HAU, Hissar.
  103. Singh, R.P., Singh, B.D. and Singh, R.N. 1990. A comparison of two breeding procedures in two crosses of mung bean (Vigna radiata (L.) Wilczek). Indian J. Genet. 50: 121-126.
  104. Singh, R.P., Singh, B.D. and Singh, I.S. 1990. Plant loss in SSD populations of mungbean (Vigna radiata (L.) Wilczek). Indian J. Genet. 50: 137-141.
  105. Singh, S.J. and Singh, B.D. 1990 Path analysis under irrigated and rainfed conditions in barley. Crop. Improv. 17: 138-140.
  106. Singh, S.J. and Singh, B.D. 1990. Genetics of earliness in barley. Crop. Improv. 17: 174-178.
  107. Singh, F., Singh, B.D. and Joshi, A.K. 1990. Evaluations of the effects of dwarfing genes on yield and yield traits in spring wheat. Egypt. J. Genet. Cytol. 19: 21-27.
  108. Singh, B.D., Singh, R.P., Singh, I.S., and Singh, K.K. 1991. Comparison of single seed descent and bulk methods and selection for yield in mung bean (Vigna radiata (L.) wilczek). In: Proc. Golden Jubilee Symp. Grain Legumes (eds. B. Sharma, R.B. Mehra, R.P. Puri, D.B. Raju, H. Ram, K. Kant and D.S. Mathur), pp. 165-176, Indian Soc. Genet. Plant Breed., IARI, New Delhi.
  109. Singh, B.D., Tewari, V. and Joshi, A.K. 1991. Genetics of leaf rust resistance in certain strains of wheat. In: Recent trends in Plant Disease Control (eds. H. B. Singh, D. N. Upadhyay and L. R. Saha), pp. 359-367. Today & Tomorrow Printers & Publishers, New Delhi.
  110. Singh, F., and Joshi, A.K. and Singh, B.D. 1991. Identification of Rht genes in certain Indian semi-dwarf wheat varieties. Crop. Improv. 18:
  111. Joshi, A.K., Singh, B.D. and Singh, F. 1992. A comparison of tall and dwarf segregants from some wheat crosses for yield and yield traits. Crop. Improv. 19: 23-28.
  112. Vaishampayan, A., Reddy, Y.R., Singh, B.D. and Singh, R.M. 1992. Reduced phosphorus requirement of a mutant Azolla- Anabaena symbiotic N2-fixing complex. J. Expl. Bot. 43: 851-856.
  113. Joshi, A.K., Singh, B.D. and Singh, F. 1993. Introgression of preharvest sprouting resistance through Rht genes in spring wheat. Seed Res. Spl. Vol: 1: 408-412.
  114. Singh, B.D. and Tiwari, K.N. 1994. Somaclonal variation in crop improvement. In: Crop Breeding in India (eds. H.G. Singh, S.N. Mishra, T.B. Singh, H.H. Ram and D.P. Singh), pp. 493-499. International Book Distributing Co., Lucknow.
  115. Arun B., Tiwari, K.N., Singh, B.D., and Dhari, Ram. 1994. High frequency embryogenesis in some elite cultivars of wheat, Triticum aestivum L. em Thell. Indian J. Expl. Biol. 32: 835-837.
  116. Maggon, R. and Singh, B. D. 1995. Promotion of bud regeneration by ABA in combination with BAP in epicotyl and hypocotyl explants of sweet orange (Citrus sinensis L. Osbeck). Scientia Hort. 63: 123-128.
  117. Chand, R., Singh, B.D., Singh, D. and Singh, P.N. 1995. Xanthomonas campestris pv. Parthenii pathovar nov. incitant of leaf blight of parthenium. Antonie von Leeuwenhoek. 68: 161-164.
  118. Dixit, G.P., Singh, B.D., and Joshi, A.K. 1995. Variability and gene action for peharvest sprouting tolerance in Indian white-seeded spring wheats. Proc. 7th Int Symp. Preharvest sprouting in cereals, Japan.
  119. Tiwari, V., Singh, B.D. and Tiwari, K.N. 1998. Shoot regeneration and somatic embryogenesis from different explants of Brahmi [Bacopa monniera (L) Wettst.]. Plant Cell Reports 17: 538-543.
  120. Singh, B.D., Tiwari, K.N., Srivastava, K., Singh, R., Shrivastava, D.K. 1998. Regeneration systems in chickpea and other grain legumes, and their possible applications. In: P. K. Gupta (ed.) Genetics and Biotechnology in Crop Improvement, pp.341-369. Rastogi Publications, Meerut. (India).
  121. Tiwari, K.N., Sharma, N.C., Tiwari, V., and Singh, B.D. 2000. Micropropagation of Centella asiatica (L.), a valuable medicinal herb. Plant Cell Tissue and Organ Culture 63: 179-185.
  122. Tiwari, V., Tiwari, K.N., and Singh, B.D. 2000. Suitability of liquid cultures for in vitro multiplication of Bacopa monniera (L.) Wettst. Phytomorphology 50: 337-342.
  123. Dixit G.P., Singh B.D., and Joshi A.K. 2001. Genetics of seed longevity in spring wheat. Indian J. Genet. 61(2): 98-100.
  124. Tiwari, V., Tiwari, K.N. and Singh, B.D. 2001. Comparative studies of cytokinins on in vitro propagation of Bacopa monniera. Plant Cell Tissue and Organ Culture 66: 9-16.
  125. Srivastava, K., Tiwari, K.N., Singh, R., Singh, B.D. and Jaiswal, H.K. 2001. Shoot regeneration from immature cotyledons of Cicer arietinum. Biol. Plant. 44: 333-337.
  126. Shrivastava, D.K., Sanyal, I., Singh, B.D. and Amla, D.V. 2001. Endogenous GUS activity in Cajanus cajan L. and some grain legumes: Selective suppression for expression of GUS reporter gene. J. Plant Biol. 28: 243-250.
  127. Singh, R., Srivastava, K., Jaiswal, H.K., Amla, D.V. and Singh, B.D. 2002. High frequency multiple shoot regeneration from decapitated embryo axes of chickpea and establishment of plantlets in the open environment. Biol. Plant. 45: 503-508.
  128. Chakrabarty, R., Viswakarma, N., Bhat, S.R., Kirti, P.B., Singh, B.D. and Chopra, V.L. 2002. Agrobacterium-mediated transformation of cauliflower: optimization of protocol and development of Bt-transgenic cauliflower. J. Biosci. 27: 495-502.
  129. Arun, B., Joshi, A.K., Chand, R. and Singh, B.D. 2003. Wheat somaclonal variants showing earliness, improved spot blotch resistance and higher yield. Euphytica 132: 235-241.
  130. Singh, N.P. and Singh, B.D. 2003. Biotechnological research in chickpea. In Masood Ali, S. Kumar and N.B. Singh (eds.) Chickpea Research in India, pp. 289-302. Indian Institute of Pulses Research, Kanpur.
  131. Tripathi, A.K., and Singh, B.D. 2004. Assessment and reduction of risks associated with genetically engineered microorganisms. In N.C. Gautam and M.P. Singh (eds.) Recent Advances in Biotechnology, pp. 147-191. Shree Publishers and Distributors. New Delhi.
  132. Singh, R.P., Prasad, B.K., Singh, S.P. and Singh, B.D. 2004. Gene transfer through somatic hybridization in brassicas. In R.C. Rajak (ed.) Biotechnology of Microbes and Sustainable Utilization, pp. 67-73. Scientific Publishers (India), Jodhpur.
  133. Vijayalakshmi, S., Yadav, K., Kushwaha, C. Sarode, S.B., Srivastava, C.P., Chand, R. and Singh, B.D. 2005. Identification of RAPD markers linked to the rust (Uromyces fabae) resistance gene in pea (Pisum sativum). Euphytica 144: 265-274.
  134. Chand, R., Srivastava, C.P., Singh, B.D. and Sarode, S.B. 2006. Identification and characterization of slow rusting components in pea (Pisum sativum L.). Genet. Resourc. Crop Evol. 53:219-214.
  135. Tiwari, V., Tiwari, K. N., and Singh, B. D. 2006. Shoot bud regeneration from different explants of Bacopa monniera (L.) Wettst. by trimethoprim and bavistin. Plant Cell Rep. 25: 629-635.
  136. Kushwaha, C., Srivastava, C.P., Chand, R., and Singh, B.D. 2007. Identification and evaluation of a critical time for assessment of slow rusting in pea against Uromyces fabae. Field Crop Res. 103: 1-4.
  137. B. Arun, B.D. Singh, S. Sharma, R. Paliwal, A.K. Joshi. 2007. Development of somaclonal variants of wheat (Triticum aestivum L.) for yield traits and disease resistance suitable for heat stressed and zero-till conditions. Field Crop Res. 103: 62-69.
  138. Kumar, S., Singh, B.D., Pandey, S., and Ram, D. 2008. Inheritance of leaf and stem morphological traits in pointed gourd (Trichosanthes dioica). J. Crop Improv. 22: 125-133.
  139. Mehrotra R., Kumar S., Mehrotra S. and Singh B.D. 2009. Seed storage protein gene regulation: A zig-saw puzzle. Indian J. Biotech. 8: 147-158.
Popular articles:
  1. B.D. Singh 1987. Krishi evam udyan krishi mein jaiva praudyogiki ke anuprayog. Vigyan Garima Sindhu 1: 25-28.
  2. B.D. Singh 1990. Bajra (Pennisetum americanum). In: Encyclopaedia Asiana. M.P. Birla Foundation, Calcutta.
  3. B. Rai and B.D. Singh 1992. Krishi, pashudhan evam vaniki ke samagra vikas mein biotechnology ka yogdan. Samagra Vikas 9: 3-5.
  4. B. Rai and B.D. Singh 1992. Jaiva Praudyogiki aur hamara jaivik bhavishya. Aviskar 22: 182-186.
  5. A.M. Kayastha and B.D. Singh 1992. Biotechnology programme at Banaras Hindu University, India. Biochem. Educ. 20: 103-105.
  6. Rai and B.D. Singh 1992. Nai kheti mein biotechnology ki ujjwal sambhavanayen. Kisan bharati 23: 6-8.
  7. B. Rai and B.D. Singh 1992. Role of biotechnology in agriculture and animal production. Indian Farmerís Digest 25: 29-31.
  8. B.D. Singh 2002. Jaiva Proudyogiki ke Naye Ayam. Vigyan 88: 1-4 & 15.

Books authored
  1. Singh, B. D. 1981. Plant Breeding: Principles and Methods. Kalyani Publishers, New Delhi (7th edition, 2005).
  2. Singh, B. D. 1990. Fundamentals of Genetics. Kalyani Publishers, New Delhi (4th edition, 2007).
  3. Singh, B. D. 1990. Padap Prajanan: Siddhant Evam Vidhiyan. Kalyani Publishers, New Delhi (now, 4th edition, 2002).
  4. Singh, B. D. 1992. Principles of Genetics. Kalyani Publishers, New Delhi.
  5. Singh, B. D. 1992. Introduction to Genetics. Kalyani Publishers, New Delhi.
  6. Singh, B. D. 2003. Anuvanshiki Ke Adhar. Kalyani Publishers, New Delhi (4th edition, 2005).
  7. Singh, B. D. 1998. Biotechnology. Kalyani Publishers, New Delhi (3rd edition, 2007).
  8. Singh, B. D. 2000. Text Book of Plant Breeding. Kalyani Publishers, New Delhi (now, 2nd edition, 2005).
  9. Singh, B. D. 2002. Objective Plant Breeding. Kalyani Publishers, New Delhi.
  10. Singh, B. D. 2003. Genetics. Kalyani Publishers, New Delhi (2nd edition, 2007).
  11. Singh, B. D. 2004. Genetic Analysis. Kalyani Publishers, New Delhi (2nd edition, 2007).
  12. Singh, B. D. 2004. Molecular Genetics. Kalyani Publishers, New Delhi (2nd edition, 2007).
  13. Singh, B. D. 2004. Biotechnology: Expanding Horizons. Kalyani Publishers, New Delhi.
  14. Singh, B. D. 2006. Plant Biotechnology. Kalyani Publishers, New Delhi.
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