(Mrs.) Shashi Pandey


Professor
Ph.D. (Univ. of Lucknow)
Contact Information:
+91 542-2307147 Ext.341 ; +91 542 6701106 ; (Fax) +91 542-2368174
Email Id:: shashipandeyrai@rediffmail.com ; shashi.bhubotany@gmail.com
Employee No. 17471

Acaedemic Qualifications:
S. No.DegreeInstitutionYear
1.B.Sc. Hons (Botany)Lucknow University1993
2.M.Sc. (Botany)Lucknow University1995
3.Ph. D. Botany ( Sp. Plant Biotechnology) CIMAP, Lucknow ,(Lucknow Univerisity)2002
 
Brief write up on area of specialization/awards/achievements:
 

Plant Molecular Biology (Genetics/Genomics aspects)

The goal of our group’s research is to genetically characterize the molecular mechanisms driving secondary metabolite biosynthesis in medicinal plants under abiotic stresses. Identifying key elements involved in these processes will allow generating novel tools for metabolic engineering of plant cells. To this end, we have developed a functional genomics platform that allows (i) comprehensive investigations of plant metabolism and (ii) gene discovery on a genome-wide scale. Broadly, transcriptome analysis of medicinal plants (Catharanthus roseus and Artemisia anuua) combined with metabolite profiling, followed by large-scale functional analysis of genes using proteomic approaches. Dr. Rai has made major contribution in the area of genetics/ genomics of regulation of stress resistance /terpenoid indole alkaloid biosynthetic pathway expression in Catharanthus roseus. For the first time she has been able to demonstrate that drought and salt resistant loss-of-function mutants simultaneously harbour pleiotropic changes in seedling and adult plant morphology. Some of the mutants harbour gross changes in the shoot morphology and inflorescence architecture, implicating involvement of chromatin remodelling in the adaptation process against abiotic stresses.

 
Contact Informations:
Assistant Professor,
Laboratory of Morphogenesis
Centre of Advance Study
Department of Botany
Faculty of Science
Banaras Hindu University (BHU),
Varanasi-221005
Tel.:0542-2307147Extno.341, 0542-6701106
Telefax: 91-0542-2368174
e-mail: shashipandeyrai@rediffmail.com ; shashi.bhubotany@gmail.com
 
Current Research Projects:
 
S. No. Name of the Project Duration Source Amount (in Rs)
1.Functional Genomic approach to prospect genes of sesquiterpene biosynthesis regulation in Artemissia annua metabolome. 2007-10DST Project No. R/Dev./ M-48-84; (PI)
2.Isolation, purification, identification and molecular assessment of microbial diversity from selected districts of Indo-Gangetic Planes. 2006-12NBAIM- ICAR; (Co-PI)
3.Role of dps protein in abiotic stress management in synacocystis sp. 2007-09INDO- JSPS Scheme DST; (Co-PI)
 
Awards & Recognition:
  1. Awarded “Birbal Sahni Memorial Gold Medal” for obtaining Ist Div. and securing the highest percentage in M. Sc. (Botany), 1995.

  2. Awarded “Shri Rajeshwar Lal Sinha and Smt. Prema Sinha Gold Medal” for obtaining highest percentage among the successful women candidates in M. Sc. Final Examination in Botany.

  3. Awarded “ Kamayani Memorial Gold Medal”, candidates in M. Sc. (Botany), 1995.

  4. Best student award in Botany from Akhil Bharti Vidyarthi Parishad, Lucknow University, Lucknow, 1995.

  5. Qualified Graduate Aptitude Test in Engineering (GATE), 1996 cunducted by IIT.

  6. Awarded, C. S. I. R. Scientist Fellowship (CIMAP), 1996, Lucknow, UP, India.

  7. Awarded C. S. I. R. Senior Research Fellowship (CIMAP), 1998-2001, Lucknow, UP, India.

  8. Awarded C. S. I. R. extended Senior Research Fellowship (CIMAP), 2001-2002, Lucknow, UP, India.

  9. Awarded C.S. I.R. Research Associate Fellowship 2002-2005, NCPGR, New Delhi.

  10. Life Member of Society of Conservation Biodiversity, India.

  11. Life Member of the Biotech Research Society, India .

  12. Life Member of the Indian Vegetable Science Society, India.

 

List of 10 major Publications:

  1. Shashi Pandey Rai, R. Luthra and Sushil Kumar (2003) Salt tolerant mutants in GLYCOPHYTIC SALINITY RESPONSE (GSR) genes in Catharanthus roseus. Theoretical and Applied Genetics 106:221-230.

  2. Ajaswrata Dutta, Jyoti Batra, Shashi Pandey-Rai, Digvijay Singh, Sushil Kumar, Jayanti Sen. (2005). Expression of terpenoid indole alkaloid biosynthetic pathway genes corresponds to accumulation of related alkaloids in Catharanthus roseus (L.) G. Don. Planta 220: 376-383.

  3. Sarika Gupta, Shashi Pandey-Rai, Suchi Srivastava, Subhas Chandra Naithani, Manoj Prasad and Sushil Kumar .Construction of primary genetic linkage map of the medicinal plant Catharanthus roseus J. Genet (2007) 86:259-68 .

  4. Shashi Pandey–Rai, Gopal Rao Mallavarapu, A A Naqvi, Anju Yadav, Sanjay Kumar Rai, Suchi Srivastava, Digvijay Singh, Raghvendra Mishra and Sushil Kumar*Volatile components of the leaves and flowers of periwinkle Catharanthus roseus (L.) G. Don grown at New Delhi. . Flavour and Fragrence (2006 ) 21, 427-430 .

  5. Digvijay Singh, Shashi Pandey-Rai, Suchi Srivastava, Sanjay Kumar Rai, Raghavendra Misra and Sushil Kumar (2004) Simultaneous quantification of some pharmaceutical Catharanthus roseus eaf and root terpenoid indole alkaloid and their precursor molecules insingle runs of a reversed-phase high performance liquid chromatographic system. Journal of AOAC International 87: (6) 1287-1296.

  6. Shashi Pandey Rai, Rajesh Luthra, M.M. Gupta and Sushil Kumar (2001) Pleiotropic morphological abiotic stress resistance of the hyper abscisic acid producing Abo- mutant in periwinkle, Catharanthus roseus. Jour. Of Biosciences 26 (1): 57-70.

  7. Sushil Kumar, Sanjay Kumar Rai, Shashi Pandey-Rai, Suchi Srivastava, Digvijay Singh (2004) Regulation of unipinnate character in the distal tendrilled domain of compound leafblade by the gene MULTIFOLIATE PINNA (MFP) in pea Pisum sativum Plant sciences 166: 929-940.

  8. Shashi Pandey Rai and Sushil Kumar (2000) Induced mutation to monocotyledony in periwinkle, Catharanthus roseus, and suppression of mutant phenotype by kinetin. Jour. Of Genetics 79 (3) : 1-8.

  9. Ruchika Tripathi, Abhijit Sarkar, Shashi Pandey Rai and S.B. Agrawal, (2009) Supplemental Ultraviolet-B and Ozone: Impact on antioxidants, proteome and genome of linseed (Linum usitatissimum L. cv Padmini) Plant Biology, (accepted).

  10. Srivastava A K , Bhargava P, Mishra Y, Rai SP, Rai LC. (2007) A rapid and cost effective method of genomic DNA isolation from cyanobacterial culture, mat and soil suitable for genomic fingerprinting and community analysis. Journal of Applied Phycology (19) 4, 373-382.

Additional Information/ Achievements:

Patent:

  1. “ Catharanthus roseus mutant plant named lli” Sushil kumar, Shashi Pandey-Rai, S.K.Rai, Suchi Srivastava, Digvijay Singh and Raghvendra Kumar Mishra. (US patent) FPO 1826 , NF28/04 granted and published online in 2006.

 

Technology Transfer:

  1. Jeevanraksha cultivars and/or processing technologies have been transferred to following companies: ALMET Corporation Limited/ Mahasrastra Hybrid Seed Company Limited, Resham Bhawan, 4th floor, 78, Veer Nariman Road, Mumbai 400020.

 

Full List of Publications:

  1. Ruchika Tripathi, Abhijit Sarkar, Shashi Pandey Rai and S.B. Agrawal, Supplemental Ultraviolet-B and Ozone: Impact on antioxidants, proteome and genome of linseed (Linum usitatissimum L. cv Padmini) Plant Biology, 2009 (accepted).

  2. M S Kanwar, BN Korla, Sushil kumar, Shashi Pandey Rai , Shachi Suchi Smita, Ram Prasad Meena, Sanjay Kumar Rai (2009) Assessing changes in character associations among biparental and f3 progenies in late cauliflower (Brassica oleracea var. botrytis l.) Journal of Vegetable Science 36 (3) 304-308.

  3. Digvijay Singh, Sanjay Kumar Rai, Shashi Pandey Rai, Suchi Srivastava, Raghvendra Kumar Mishra, Swati Chaudhary, Srikant Sharma and Sushil Kumar (2008) predominance of serpentine route in monoterpenoid Indole alkaloid pathway of Catharanthus roseus. PINSA 74 (3) 97-109.

  4. Srivastava A K , Bhargava P, Mishra Y, Rai S.P., Rai LC. (2007) .A rapid and cost effective method of genomic DNA isolation from cyanobacterial culture, mat and soil suitable for genomic fingerprinting and community analysis. Journal of Applied Phycology 19, ( 4), 2007, 373-382.

  5. Sarika Gupta, Shashi Pandey-Rai, Suchi Srivastava, Subhas Chandra Naithani, Manoj Prasad and Sushil Kumar (2007) Construction of primary genetic linkage map of the medicinal plant Catharanthus roseus J. Genet, 86:259-68 .

  6. Shashi Pandey–Rai, Gopal Rao Mallavarapu, A A Naqvi, Anju Yadav, Sanjay Kumar Rai, Suchi Srivastava, Digvijay Singh, Raghvendra Mishra and Sushil Kumar*Volatile components of the leaves and flowers of periwinkle Catharanthus roseus (L.) G. Don grown at New Delhi. . Flavour and Fragrence (2006 )21, 427-430 .

  7. Sanjay Kumar Rai, Gopal Rao Mallavarapu, Shashi Pandey-Rai, Suchi Srivastava, Digvijay Singh, Raghvendra Mishra and Sushil Kumar Constitution of the flower oil of Carissa opaca growing in Aravalli mountain range at New Delhi. Flavour and Fragrance (2006) 21 , 304-305.

  8. Suchi Srivastava, Gopal Rao Mallavarapu, Sanjay Kumar Rai, Digvijay Singh, Raghvendra Mishra, Shashi Pandey-Rai and Sushil Kumar Composition of the essential oils of the leaves and flowers of Rhus musurensis Heyne ex Weight &Arn growing in Aravalli mountain range at New Delhi. Flavour and Fregrance (2006) 21, 228-229.

  9. Ajaswrata Dutta, Jyoti Batra, Shashi Pandey-Rai, Digvijay Singh, Sushil Kumar, Jayanti Sen. (2005). Expression of terpenoid indole alkaloid biosynthetic pathway genes corresponds to accumulation of related alkaloids in Catharanthus roseus (L.) G. Don. Planta 220: 376-383.

  10. Digvijay Singh, Shashi Pandey-Rai, Suchi Srivastava, Sanjay Kumar Rai, Raghavendra Misra and Sushil Kumar (2004) Simultaneous quantification of some pharmaceutical Catharanthus roseus eaf and root terpenoid indole alkaloid and their precursor molecules insingle runs of a reversed-phase high performance liquid chromatographic system. Journal of AOAC International 87: (6) 1287-1296.

  11. 11. Sushil Kumar, Sanjay Kumar Rai, Shashi Pandey-Rai, Suchi Srivastava, Digvijay Singh (2004) Regulation of unipinnate character in the distal tendrilled domain of compound leafblade by the gene MULTIFOLIATE PINNA (MFP) in pea Pisum sativum Plant sciences 166: 929-940.

  12. Shashi Pandey Rai, R. Luthra and Sushil Kumar (2003) Salt tolerant mutants in GLYCOPHYTIC SALINITY RESPONSE (GSR) genes in Catharanthus roseus. Theoretical and Applied Genetics 106:221-230.

  13. Kumar S. Pandey Rai S and Rai SK (2003) Research and development approaches for advancing the contribution of medicinal and aromatic plant commodities to increase profit of farmers, generate new jobs and improve availability of fragrance , flavour and therapeutic materials J of Med. and Arom. Plant Sci (proc.) pp 20-23.

  14. Sushil Kumar, Ashok Kumar, Sanjay Kumar Rai and Shashi Pandey Rai (2003) Trends in plant medicinal research and development and scope of medicines –a review .Vijnana Parishad Anushandhan Patrika, Vol. 46, 100-118.

  15. Shashi Pandey Rai, Rajesh Luthra, M.M. Gupta and Sushil Kumar (2001) Pleiotropic morphological abiotic stress resistance of the hyper abscisic acid producing Abo- mutant in periwinkle, Catharanthus roseus. Jour. Of Biosciences 26 (1): 57-70.

  16. Shashi Pandey Rai and Sushil Kumar (2001) Hetrocarpous flowers resulting from a recessive mutation in periwinkle, Catharanthus roseus. Current Science (India) 80: 1581-1584.

  17. Shashi Pandey Rai and Sushil Kumar (2000) Induced mutation to monocotyledony in periwinkle, Catharanthus roseus, and suppression of mutant phenotype by kinetin. Jour. Of Genetics 79 (3) : 1-8.

  18. Shashi Pandey Rai and Sushil Kumar (2000) Loss of negative control on rooting in hyper rooting Rrg- mutant of periwinkle, Catharanthus roseus. J of Med. and Arom. Plant Sci. 22 (4A) and 23 (1A): 300-302.

  19. Shashi Pandey Rai and Sushil Kumar (2000) A tricotyledonous seedling mutant with Mendelian inheritance in periwinkle, Catharanthus roseus. J of Med. and Arom. Plant Sci. 22 (4A) and 23 (1A): 267-268 .

  20. Shashi Pandey Rai, R. Luthra and Sushil Kumar (2000) Differential expression of proteins in the monogenic salt resistant mutants with and without thermal stress in periwinkle, Catharanthus roseus. J of Med. and Arom. Plant Sci. 22 (4A) and 23 (1A): 287-290 .

  21. Shashi Pandey Rai, Srikant Sharma and Sushil Kumar (2000) Pleiotropic variation in the induced mutants possessing abnormal morphology in periwinkle, Catharanthus roseus. J of Med. and Arom. Plant Sci. 22 (4A) and 23 (1A): 252-258 .

  22. S.K.Rai, R.S. Katiyar, Shashi Pandey-Rai ,and S.P. Singh (2000) Nitrogen ,phasphorus and potash urverkon ka chayadar sthalon mein chaya pralobhi Adarak ki kheti per prabhav.(In Hindi) In Proc. Akhil Bhartiya Vigyan Sammelan- 2000.

 

Books Reviews/Chapters:

  1. Sushil Kumar, Pandey-Rai S., Rai S.K. Mint cultivation in India (2006) in Mint: The Genus Mentha edited by Brian M. Lawrence Journal of Essential Oil Research, Winston Salem, NC, USA Series: Medicinal and Aromatic Plants - Industrial Profiles Volume: 44 .

  2. Sanjay Kumar Rai, Sushil Kumar ,Shachi Shuchi Smita ,Manoj Kumar Pandey, Ram Prasad Meena and Shashi Pandey-Rai, (2010)Molecular map based breeding and descriptor’s suitable for mapping in Catharanthus roseus for valuable pharmaceutical alkaloid yields Plant Genome Biodiversity, Conservation and Manipulation ( Accepted).

  3. Shashi Pandey-Rai, Shachi Suchi Smita and *S.K. Rai, Ploidy : (2010) A prime facilitator of speciation and evolution in plants. Plant Genome Biodiversity, Conservation and Manipulation ( Accepted).

 

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