(a) Defining molecular targets for anticancer agents in in vivo tumor models
We have (a) characterized > 4 novel Ru (II) complexes and fisetin & emodin as modulators of Akt-COX2-EP1-apoptosis and Akt-HI1α-bioenergetic-apoptotic pathways vis a vis regression of Dalton’s lymphoma and hepatocellular carcinoma in vivo
(b) Neurobiology of Hepatic-encephalopathy (HE):
We have developed CLF model of HE and demonstrated involvement of glutamate-NMDAR-nNOS pathway in the cortex and cerebellum during pathogenesis of chronic HE. Glutamate-NMDAR-pannexin-1 & NMDAR-SIRT-1 signaling is the current focus of research in this area.

Awards
- AMBO fellowship, 2000
- BOYSCAST Fellowship of DST, 2002,
- Senior Scientist Medal of ICCB, 2009
- Member, Editorial/Reviewer Board of Scientific Journals: 03
(a) Journal of Paediatric Neurology
(b) AJBR (African Journal of Biochemistry Research)
(c) Scientific Journals International (SJI)
- Elected/Nominated for higher academic bodies of Istitutions/Scientific organizations :
(b) Member, Advisory Board: IBAS (Inst. Biotech. & Allied sciences), Sikar : 2009
(c) Served as Secretary and Executive council member of AGI
(d) Member, Executive Council: Association of Gerontology (India): 2009
(e) Member, Advisory Board: SNT Memorial Research Foundation: 2005-0nward
(f) Member advisory Board, Ranchi University, 2009
(g) Chaired Scientific Sessions in many National and International conferences
(h) Presented invited & plenary lectures
(i) Serving mentor for DST - INSPIRE programmes.
(j) Serving as Chairman, UACC, Institute of Science BHU; Chairman, ARS, BHU
(k) Served as Student Advisor, faculty of Science, BHU
(l) Served as Chairman, Student Council Election-2014, BHU
(m) Chaired scientific sessions and presented Plenary/Invited Lectures in many national and international Conferences

List of 10 best Publications:

1. Khanna A, Trigun SK (2016) Resveratrol normalizes hyperammonemia induced pro-inflammatory and pro-apoptotic conditions in rat brain. International Journal of Complementary & Alternative Medicine (in press)
2. Singh S, Mondal P, Trigun SK (2014) Acute Liver Failure in Rats Activates Glutamine- Glutamate Cycle but Declines Antioxidant Enzymes to Induce Oxidative Stress in Cerebral Cortex and Cerebellum. PLoS ONE 9(4): e95855. doi:10.1371/journal.pone.0095855
3. Mondal P, Trigun SK (2014) Pannexin1 as a novel cerebral target in pathogenesis of hepatic encephalopathy. Metabolic Brain Diseases, 29: 1007-1015DOI: 10.1007/s11011-014-9556-x
4. Singh S, Trigun SK (2014) Low grade cirrhosis induces cognitive impairment and motor dysfunction in rats: Could be a model for minimal hepatic encephalopathy. Neurosci. Lett. 559:136-140
5. Mehrotra A and Trigun SK. (2012) Moderate grade Hyperammonemia induced concordant activation of antioxidant enzymes is associated with prevention of oxidative stress in brain slices. Neurochemical Research, 37: 171-181.
6. Singh S, Trigun SK (2010) Activation of neuronal nitric oxide synthase in cerebellum of chronic hepatic encephalopathy rats is associated with up-regulation of NADPH- producing pathway. Cerebellum, 9:384-397.
7. Maurya BK, Trigun SK (2016) Fisetin modulates antioxidant enzymes and inflammatory factors to inhibit aflatoxin-B1 induced hepatocellular carcinoma in rats. Oxidative medicine and Cellular Lonevity ID 174839
8. Koiri RK, Trigun SK, Mishra L (2015) Activation of p53 mediated glycolytic inhibition-oxidative stress-apoptosis pathway in Dalton’s lymphoma by a ruthenium (II)-complex containing 4-carboxy N-ethylbenzamide. Biochimie, 110: 52-61.
9. Singh KB, Maurya BK, Trigun SK (2015) Activation of oxidative stress and inflammatory factors could account for histopathological progression of Aflatoxin- B1induced hepatocarcinogenesis in rats. Moecular and Cellular Biochemistry, 401:185-196.
10. Koiri RK, Trigun SK, Mishra L, Pandey K, Dixit D, Dubey SK (2009) Regression of Dalton’s lymphoma in vivo via decline in lactate dehydrogenase and induction of apoptosis by a Ruthenium (II)-complex containing 4-carboxy N-ethylbenzamide as ligand. Investigational New Drugs, 27: 503-516.

A. Full List of the Publications:

1. Khanna A, Trigun SK (2016) Resveratrol normalizes hyperammonemia induced pro-inflammatory and pro-apoptotic conditions in rat brain. IJCAM (Int. J. Complement Alt Med) (In press).
2. Maurya BK, Trigun SK (2016) Fisetin modulates antioxidant enzymes and inflammatory factors to inhibit aflatoxin-B1 induced hepatocellular carcinoma in rats. Oxidative medicine and Cellular Lonevity ID 174839
3. Mondal P, Trigun SK (2015) Bacopa monnieri extract (CDRI-08) modulates the NMDAR receptor subunits and nNOS-apoptosis axis in cerebellum of hepatic encephalopathy rats. ECAM: Evidence-based Complementary and Alternative Medicine, ID: 535013
4. Koiri RK, Trigun SK, Mishra L (2015) Activation of p53 mediated glycolytic inhibition-oxidative stress-apoptosis pathway in Dalton’s lymphoma by a ruthenium (II)-complex containing 4-carboxy N-ethylbenzamide. Biochimie, 110: 52-61
5. Singh KB, Maurya BK, Trigun SK (2015) Activation of oxidative stress and inflammatory factors could account for histopathological progression of Aflatoxin-B1induced hepatocarcinogenesis in rats. Mol. Cell Biochem. 401:185-196.
6. Kumar A, Gupta RK, Kumar A, Paitandi RP, Singh KB, Trigun SK, Hundal MS, Pandey DS (2015) Cataioic Ru(II), Rh(III) and Ir(III) complexes containing cyclic perimeter and 2- aminophenyl benzimidazole ligands: Synthesis, molecular structure, DNA and protein binding, cytotoxicity and anticancer activity. J. Organometalic Chemistry
7. Singh S, Mondal P, Trigun SK (2014) Acute Liver Failure in Rats Activates Glutamine-Glutamate Cycle but Declines Antioxidant Enzymes to Induce Oxidative Stress in Cerebral Cortex and Cerebellum. PLoS ONE 9(4): e95855.doi:10.1371/journal.pone.0095855
8. Mondal P, Trigun SK (2014) Pannexin1 as a novel cerebral target in pathogenesis of hepatic encephalopathy. Metabolic Brain Diseases, 29: 1007-1015DOI: 10.1007/s11011-014-9556-x
9. Singh S, Trigun SK (2014) Low grade cirrhosis induces cognitive impairment and motor dysfunction in rats: Could be a model for minimal hepatic encephalopathy. Neurosci. Lett. 559:136-140
10. Mehrotra A and Trigun SK (2013) Moderate grade hyperammonemia activates lactate dehydrogenase-4 and 6-phosphofructo-2-kinase to support increased lactate turnover in the brain slices. Mol Cell Biochem. DOI: 10.1007/s11010-013-1698-3.
11. Koiri RK, Mehrotra A, Trigun SK (2013) Targetting cancer with Ru(III/II)- phosphodiesterase inhibitor adducts: A novel approach in the treatment of cancer. Medical. Hypothesis. 80: 841-846
12. Mehrotra A and Trigun SK. (2012) Moderate grade Hyperammonemia induced concordant activation of antioxidant enzymes is associated with prevention of Oxidative stress in brain slices. Neurochemical Research. 37: 171-181
13. Singh KB, Trigun SK (2012) Apoptosis of Dalton’s lymphoma due to in vivo treatment with emodin is associated with modulations of hydrogen peroxide metabolizing antioxidant enzymes. Cell Biochem. Biophys. DOI 10.1007/s12013-011-9305-2.
14. Koiri RK, Trigun SK (2011) Dimethyl sulphooxide activates tumor nectrosis factorα-p53 mediated apoptosis and down regulates D-fructose-6-phosphate-2-kinase and lactate dehydrogenase-5 in Dalton’s lymphoma in vivo. Leuk. Res. 35: 950-956
15. Dixit N, Koiri RK, Maurya BK, Trigun SK, H¨obartner C, Mishra L (2011) One pot synthesis of Cu(II) 2,2’-bipyridyl complexes of 5-hydroxy-hydurilic acid and alloxanic acid: Synthesis, crystal structure, chemical nuclease activity and cytotoxicity. J. Inorg. Biochem. 105: 256- 267.
16. Kumari N, Maurya BK, Koiri RK, Trigun SK, Saripella S, Coogan MP and Mishra L. (2011) Cytotoxic activity, cell imaging and photocleavage of DNA induced by a Pt (II) Cyclophane bearing 1,2 diamino ethane as terminal ligand. Med.Chem.Commun.2, 1208- 1216.
17. Singh S, Trigun SK (2010) Activation of neuronal nitric oxide synthase in cerebellum of chronic hepatic encephalopathy rats is associated with up-regulation of NADPH- producing pathway. Cerebellum, 9:384-397
18. Prajapati R, Dubey SK, Gaur R, Koiri RK, Maurya BK, Trigun SK, Mishra L (2010) Structural characterization and cytotoxicity studies of Ruthenium(II)-dmso-chloro complexes of chalcone and flavone derivatives. Polyhedron, 29: 1055-1061
19. Koiri RK, Trigun SK, Mishra L, Pandey K, Dixit D, Dubey SK (2009) Regression of Dalton’s lymphoma in vivo via decline in lactate dehydrogenase and induction of apoptosis by a Ruthenium (II)-complex containing 4-carboxy N-ethylbenzamide as ligand. Invest New Drugs, 27: 503-516
20. Singh S, Koiri RK, Trigun SK (2008) Acute and chronic hyperammonemia modulate antioxidant enzymes differently in cerebral cortex and cerebellum. Neurochem. Res. 33: 103-113
21. Koiri RK, Trigun SK., Dubey SK, Mishra L (2008), Metal Cu(II), Zn(II) Bipyridyls as inhibitors of Lactate dehyrogenase. BioMetals 21: 117-126
22. Shukla VK, Goel S, Trigun SK, Sharma D (2008) Electrophoretic pattern of proteins in carcinoma of the gallbladder. Eur. J. Cancer Prevention. 17: 9-12
23. Trigun S.K, Koiri R, Mishra L, Dubey S.K, Singh S, Pandey P. (2007)Ruthenium commplexes as Enzyme modulator: Modulation of Lactate Dehydrogenase by a Ruthenium (II) complex containing 4-carboxyl N-ethylbenzamide as ligand, Curent Enzyme Inhibition. 3: 243-253.
24. Singh S, Trigun SK (2007) Differential Expression of Antioxidant and Nitrosative stress enzymes in cerebral cortex and cerebellum of rats with chronic liver cirrhosis (Ab). Ann. Neurosci. 14: 107
25. Trigun SK., Singh AP, Asthana RK, Pandey SM., Pandey , P, Singh, SK., Singh, S P (2006) Assesment of Bioactivity of Fischerella species colonizing Azadirechta indica (neem) bark. Appl. Ecol. Envirn. Res. 4: 119-128
26. Trigun S.K, Pandey P, Singh S, Koiri R.K (2006) Brain metabolism adapts positively against most of the unphysiological challenges (Ab), Ann. Neurosci. 13: 47.
27. Pandey P, Singh S.K, Trigun S.K (2005) Fructose-2,6-bisphosphate associated regulatory enzymes develop in concordance in mice brain during early postnatal life. Neurol. Psych. Brain Res. 12: 69-74.
28. Pandey P, Singh SK, Trigun SK (2005) Developing brain of moderately hypothyroid Mice shows adaptive changes in the key enzymes of glucose metabolism. Neurol. Psych. Brain Res. 12: 159-164.
29. Mishra L, Singh A.K, Trigun S.K, Singh S.K and Pandey S.M (2004) Anti HIV and cytotoxic Ruthenium (II) complexes containing flavones : Biochemical evaluation in mice. Ind. J. Exptl. Biol 42: 660-666
30. Srivastava A.S, Trigun S.K. and Singh S.N. (1997) Activity of cytosolic and mitochondrial Alanine aminotransferase during pre-spawning phase of air- breathing and non-air-Breathing fresh water fishes. J. Scientific. Res. 47, 163-171.
31. Tamaru T, Trigun S.K, Okada M. and Nakagawa H. (1993) Identification of cells Expressing a D type G1 cycline in mature brain: Implication for the role in neuronal functions. Neuroscience Lett. 153, 169-172.
32. Trigun S.K. and Singh S.N. (1989) Evidence for tissue specific alterations in Zn2+- induced conformational changes in fructose-bisphosphatase of senescent rats. J. Inorg. Biochem. 35, 255-266.
33. Trigun S.K. and Singh S.N. (1989) Age-linked alterations in fructose-2,6-bisphosphate induced modulations of rat muscle phosphofructokinase. Biochem. Intl. 19, 1213- 1221. IUBMB Life
34. Trigun S.K. and Singh S.N. (1988) Comparison in the molecular properties of muscle phosphofructokinase of young and old rats. Cell. Molec. Biol. 34, 207-213.
35. Trigun S.K. and Singh S.N. (1988) Changes in the modulations of kinetics and allosteric properties of muscle phosphofructokinase of young and old rats. Arch. Gerontol. Geriatr. 7, 239- 247.
36. Trigun S.K. and Singh S.N.(1988) Influence of age on the conformation of rat muscle phosphofructokinase: Analysis of pH-dependent kinetics and limited proteolysis of the enzyme. Ind. J. Exptl. Biol. 26, 532- 534
37. Trigun S.K. and Singh S.N. (1987) Purification and age-related comparison in molecular properties of unproteolysed fructose-1,6-bisphosphatase of skeletal muscle of young and old rats. Biochem. Arch. 3, 343-351.
38. Trigun S.K. and Singh S.N. (1987) Alterations in the sensitivity to endogenous proteolysis due to storage of fructose-1,6-bisphosphatase extracts of liver and muscle of young and old rats. Compr. Gerontol. 1, 87-90.
39. Trigun S.K. and Singh S.N. (1987) In vitro studies on regulatory properties of muscle phosphofructokinase of young and old rats. Biochem. Arch. 3, 477-484.
40. Trigun S.K. and Singh S.N. (1987) Differential Expressions of Phosphofructokinase isozymes in different tissues of young and old rats. Cell. Molec. Biol. 33, 767-774.
    B. Book Chapter:
    1. Trigun S.K and Singh S.N. (1990) Indication of age-dependent conformational alterations in rat muscle PFK : Analysis by reversible oxidation of the enzyme. In. Perspectives in aging research. (Eds. Singh, R, & Singhal, G.S. ) Today & Tomorrow Printers and Publishers, New Delhi. Pp. 81-85 .
    2. Singh S.N. and Trigun S.K. (1991) Age-related alterations and modulations of cyytosolic Enzymes of liver of rats. In. Liver and Aging (Ed. Kitani, K.), Elsevier Science Publ. BV, Amsterdam, p. 253-262.
    3. Srivastava, A.S., Trigun, S.K. and Singh, S.N. (2000) Activity of cytosolic and mitochondrial Aspartate aminotransferase during pre-spawning phase of air- breathing and non-air-Breathing fishes. In. Chronobiology : (Ed. M. Shedpure), Univ. Press, Raipur, pp. 99-109.